Illustration from Scientific Research

Side-by-side comparison of the Rad24 upper loop (green) and Rfc5 plug (cyan) interacting with the 5-nt gapped DNA (left) and the 10-nt gapped DNA from step 5 (right). Note that the lower 3' dsDNA in the left structure is rotated by 180° around its helical axis such that the purple template strand is oriented to connect with the upper 5' dsDNA with a minimum length (5 nt).

DOI