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DNA oligos were annealed with a longer template DNA to provide either an isolated 5' end (primer #2 only) or an ssDNA gap of various lengths (both primers #1 and #2; seeTable S1). The32P-9-1-1 clamp is blocked from sliding by the biotin (attached to magnetic streptavidin beads) on one end and the DIG moiety attached to the Fab of an antibody to DIG at the other end. SeeSTAR Methodsfor details.

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