Biosynthetic reactions with path preference. For each observed addition of two monosaccharides in diamond-shaped network motifs, we grouped their probabilities across glycan sequences (i.e., monosaccharides A and B could be added to glycan X, Y, Z, etc.) in both the forward (A-then-B) and reverse (B-then-A) order. Shown are additions with a mean difference of at least 15 occurring in more than two sequences. Mean difference was tested via two-tailed Welch’s t tests. Data are depicted as mean values, with box edges indicating quartiles and whiskers indicating the remaining data distribution. Dots indicate outlier data points outside the whiskers.p < 0.001;p < 0.01;p < 0.05; n.s. (not significant), p > 0.05.
