Schematics illustrating ventral (A(i)) mEC regions respectively. Representative confocal brain slice images containing dorsal and ventral mEC layer II stellate neurons are shown (seeFigure S1), with the morphologies of these neurons displayed in the insets. The scales in (A(i)) represent 500 μm and 20 μm. Typical electrophysiological recordings from (A(ii)) wild-type (WT) neurons when the protocol shown was applied under control conditions (con) and after TTA-P2 application. The membrane potential is noted next to each trace. The scale associated with the first panel applies to all panels. The graphs on the right portray the mean ± SEM action potentials (AP no.) elicited by applying current injections in the absence (con), presence, and following washout of TTA-P2 in seven dorsal and ventral WT neurons as indicated in brackets. Paired t tests were used to determine significance (p) values (exact values inTable S7).
