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Knockdown and overexpression of BHLHE40 in primary mouse hepatocytes resulted in a transcriptional (qPCR) up- and downregulation, respectively, ofBhlhe41. Primary mouse hepatocytes with siControl and siBhlhe40 or infected with adenoviruses expressing GFP or BHLHE40 were exposed to 25 mM glc for 72 h (n = 4). Student’s t test (two-tailed) was used for statistics.

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