Scatter Plot from Scientific Research

Quantification of cell division angle in MCF10A ER-RasV12-Tubulin-GFP spheroids relative to the spheroid edge. The spheroid and division axes were manually segmented as illustrated in (I). A line from spheroid centroid to the periphery intersecting the dividing cell and a line parallel to the anaphase chromosomes were drawn. The Feret angle (0 = x axis) is calculated for each, and from these, quantification of the division axis relative to the spheroid axis is calculated. P values calculated using Mann-Whitney test. N = 2 experiments. See alsoVideos S3andS4.

DOI