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Schematic to illustrate the mitotic shake-off protocol. ER-RasV12cells were treated with 10 mol S-trityl-L-cysteine (STLC) and ethanol or tamoxifen for 15 h prior to mitotic shake-off. Mitotic cells are then dislodged by mechanical force into suspension. Upon removal of STLC, cells are then replated onto fibronectin -coated glass dishes in ethanol or tamoxifen-containing media and immediately imaged using bright-field time-lapse microscopy at 5-min intervals as they exit mitosis.

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