Line Plot from Scientific Research

Cell outlines as cells respread post-mitosis with respect to the mother cell shape. MCF10A-LifeAct-GFP-ER-RasV12cells were treated with ethanol, 4-OHT, or 4-OHT plus 10 M selumetinib for 5-15 h prior to time-lapse bright-field and fluorescence imaging at 5-min intervals. Representative dividing cells were manually segmented at interphase (black), defined using the bright-field channel at 15 min before nuclear envelope breakdown (NEB). Post-mitotic respreading cell outlines were segmented at 5-min intervals for 10 consecutive frames from the final frame of metaphase following ethanol (blue), 4-OHT (red), or 4-OHT plus MEKi (green). Image overlays were produced using CellProfiler software as described inSTAR Methods.

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