Comparison of experimental and simulated pore aperture response in stomata induced to open or close via Fc or ABA (experimental) or the extremes of the intact reciprocal pressure model shown in (D) (simulated). Points indicate individual stomata for experimentally measured data and individual models derived from independently derived FEMs for simulated data. Horizontal lines indicate median values, with box plots indicating the range of the 25th–75thpercentile. Pore area was significantly greater in open compared with closed stomata in both cases (Kruskal-Wallis, H = 60.85, n = 24, 24, 20, and 20; p≤0.0001), but it was not possible to distinguish experimental and simulated data following Dunn’s multiple comparisons test (p ≤ 0.05), with letters indicating samples that cannot be distinguished from each other.
