There is a more prominent dip in the ACCp signal (average signal from 0 to +5 s following a fentanyl reward minus average signal at pre-reward baseline, -5 to 0 s) during fentanyl bouts than during consumption of quinine or single fentanyl rewards. A similar pattern, albeit with modest signal deflections, is apparent for quinine. Liquid, p = 0.0130, F(1, 4) = 18.230; is bout, p = 1.2e-04, F(1, 4) = 222.709; interaction, p = 0.0174, F(1, 4) = 15.291; ANOVA on LMM of delta signal ~ liquid*is bout|animal, n = 5 mice. *p < 0.05, ***p < 0.001; error bars and shaded errors represent SEM. See also Figure S2 and Table S1.
