Distribution of ACCp and OFCp claustrum projection neurons as a fraction of Fos+cells versus total claustral cells. Of all cells, 7% ± 1% project only to ACC, 8% ± 1% project to both ACC and OFC, and 21% ± 1% project to OFC. Of Fos+ cells, 13% ± 1% project only to ACC, 17% ± 1% project to both ACC and OFC, and 30% ± 2% project to OFC. ACCp only, p = 0.0011, F(1, 5) = 45.212; ACCp and OFCp, p = 0.0002, F(1, 5) = 95.837; OFCp only, p = 0.0024, F(1, 5) = 32.141; non-frontal-projecting cells, p = 2.2e−05, F(1, 5) = 231.209). ANOVA on LMM of %projection ~ reference total (of DAPI or ofFos) +AP location|animal; fentanyl n = 6 mice, 70 sections, saline n = 5 mice, 56 sections.*p < 0.05,**p < 0.01,***p < 0.001. See alsoFigure S1andTable S1.
