Microscopy from Scientific Research

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Maximum-intensity projections from a time lapse following wounding of a 3-dpf larva expressing mCherry and GFP-2xFYVE in the basal cells that were wounded in the presence of E3 media:(TgBAC(Np63:Gal4); Tg(UAS:mCherry); Tg(UAS:2xFYVE-GFP)). First and second columns show the mCherry and GFP-FYVE channels, respectively. Third column shows the merge. Fourth column shows segmentation of the GFP-FYVE. The segmented structures were divided into three categories and pseudocolored (orange: < 1 m2; blue: 1-5 m2; and black: >5 m2). Red arrowheads show large endocytic structures. Full sized images are shown in Figure S4.
#Microscopy#Maximum-intensity Projections#Time Lapse#Larva#mCherry#GFP-2xFYVE#Basal Cells#Segmentation#Endocytic Structures
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