Heatmaps of cell area extension (D) and proliferation (F) in control and RCOcds-YPet induced leaves between 1 DAH and 4 DAH. Note a reduction in cell proliferation and area extension in RCOcds-YPet-expressing clones, compared with cells at an equivalent position in control samples (compare heatmap intensity in cells outlined with dotted polygons in control and induced). Quantification of cell area extension (E) and cell proliferation (G) in control and RCOcds-YPet induced leaves between 1 DAH and 4 DAH. n = 9 RCOcds-YPet clones from 3 induction and time-lapse experiments; each RCOcds-YPet clone compared against 3 non-RCO-expressing clones at equivalent position in control (n = 3 time-lapse experiments). White arrowheads in (D) and (F) indicate relatively increased cell growth and proliferation in the neighboring cells of RCOcds-YPet-expressing clones than the RCOcds-YPet-expressing clones (see alsoFigures S4I and S4J). Statistical significance was tested using a linear regression model in (E) and a generalized linear regression model in (G) with a significance threshold ofP< 0.05. Scale bars, 50 m (A–C), (D), and (F). DAH, days after heatshock. See alsoFigures S4andS5.
