Macro-F1 and AMI scores were computed using the manually annotated hypothalamic nuclei in b . Each point represents the performance on a given single-cell spatial image; the horizontal bars represent the mean across n = 5 images. Performances (points) on the same images are connected by gray dashed lines. P values were computed using a one-sided paired t -test. 3V, third ventricle; BAC, bed nucleus of the anterior commissure; Fx, fornix; LPO, lateral preoptic area; PS, parastrial nucleus; PVA, paraventricular thalamic nucleus; StHy, striohypothalamic nucleus.