Workflow from Scientific Research

Open access visualization of Workflow, Illustration, p16-FDR allele, Cdkn2a, P2A
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DOI: 10.1016/j.ccell.2023.05.004

DOI

Targeting strategy to generate the p16-FDR allele. Exons 1-3 of Cdkn2a are shown. P2A, 2A self-cleaving peptide; FLPo, mammalian optimized FLP-recombinase; HA, homology arm. Red arrows show primers used to screen for correct insertion of the FDR cassette by PCR and Sanger sequencing.

#Workflow#Illustration#p16-FDR allele#Cdkn2a#P2A#FLP-recombinase#Homology arm#PCR#Sanger sequencing

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