Microscopy from Scientific Research

HeLa cells were cultured in basal conditions or with FBS-depleted media in presence or absence of CCL5 (50 nM) for 4 h before fixation. Representative confocal micrographs and quantification of cells immunostained with anti-RAB11 (green) and anti-LC3 (red) antibody. High magnification insets are shown. Confocal micrographs of separate channels are included inFigures S7I and S7J. Plots represent mean SEM. (n = 3 independent experiments, n = 5–7 randomly selected fields/condition, each containing ~ 10 cells) Two-tailed, paired Student’s t test*p < 0.05,**p < 0.01, ns not significant. Scale bars, 10 m.

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