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HeLa cells were starved for 1 h, loaded with ferrofluid conjugated with anti-TfR Ab or anti-CCR5 Ab for 1 h, and chased for 15 min in starvation medium. The cells were then fragmented and membranes containing ferrofluid conjugated with anti-TfR or anti-CCR5 Ab (B = bound) or not containing ferrofluid (U = unbound) were separated and processed for immunoblotting.
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