Bar Plot from Scientific Research

HeLa cells were cultured in basal or FBS-depleted media for 10 h and processed by proximity ligation assay (PLA) using anti-FLAG and anti-LC3 Abs. Representative confocal micrographs and quantification of FLAG-CCR5 and endogenous LC3 interaction by PLA are shown. Plotted data represent mean SEM (n = 3 independent experiments, n = 5–7 randomly selected fields/condition, each containing ~10 cells). One-tailed, paired Student’s t test*p < 0.05. Nuclei were counterstained with DAPI. Scale bars, 20 M.

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