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Western blotting and densitometry analyses of LC3-II protein levels in mouse primary neurons treated as described in (A) and incubated in DMSO or 400 nM bafilomycin A1 (BAF) for 8 h before being lysed. TUBULIN was loading control. Plots represent mean ± SEM (n = 4 independent experiments).Two-tailed, one-sample t test*p < 0.05, ns, not significant.

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