Workflow from Scientific Research

Schematic of conditioned media used in experiments in (F). CCL3, CCL4, and CCL5 were immunodepleted from conditioned media derived from BV2 cells, previously activated by LPS (10 ng/mL) +INFγ (100 ng/mL), using specific IgG-anti-CCL3/-4/-5. Neurobasal media supplemented with LPS+INFγ or conditioned media from activated BV2 cells were incubated with non-specific IgG and used as controls.

DOI