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Schematic of experimental configuration for fiber photometry in SC in a mouse running on a treadmill. Two excitation wavelengths at 470 nm (blue; excitation wavelength) and 405 nm (violet; isosbestic hemodynamic control channel) are alternated while imaging visual responses of NTSR1-GN209-Cre-tagged neurons conditionally expressing GCaMP7f. The animal is presented with a dot which progresses along a naso-temporal trajectory either at a speed commensurate to the running speed of the mouse (coupled), or, on alternate trials, with a dot that replays the previous coupled stimulus irrespective of the running speed of the mouse (uncoupled).

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