Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Experimental Configuration, Optrode, Neuronal Activity
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Schematics of experimental configuration. An optrode is used to silence neuronal activity in SC by optogenetically activating GABAergic neurons conditionally expressing ChR2 in a GAD-Cre mouse. Electrophysiological recordings are performed simultaneously in visuotopically matching regions of V1 and lateral HVAs. The visual stimulus consists of a drifting grating (diameter: 45). The recordings in the lateral HVAs were centered around LI near its borders with POR and LM. The stereotactic injection of the anterograde tracer AAV1.CAG.tdTomato (red) in V1 enabled the identification of the areas LI, POR and LM.

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