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The sera from four individual mice at each time point were used in ELISAs. Mice 2-1 and 2-4 were immunized with wild-type Che8, while mice 3-2 and 3-4 were immunized with Che8 110-1, as indicated above each dataset (see alsoTable S3). Logistic fits of the ELISA curves yield half-maximal serum titers of antibodies that bind to both wild-type Che8 (red) and Che8 110-1 (blue), as well as uncoated wells (black).
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