Histogram from Scientific Research

Choice of targeted mutations based on measured effects in prior deep mutational scanning29and occurrences in natural HIV sequences. The distributions of previously measured mutation effects are shown for all mutations to BF520 (black) with highlighting of subsets of mutations (purple). From left to right, highlighted are mutations well tolerated in the prior deep mutational scanning,29mutations found multiple times in natural sequences, and the union of these two sets. Mutations in the union of the two sets were designed into our libraries.

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