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The lentivirus genome used for deep mutational scanning. The genome contains the full 5' and 3' LTR sequences, including the U3 sequence usually deleted in the 3' LTR. Env is under control of an inducible TRE3G promoter and followed by a 16N random nucleotide barcode. A CMV promoter drives ZsGreen and puromycin resistance (PuR) expression.
#Workflow#Lentivirus Genome#Deep Mutational Scanning#LTR Sequences#Inducible TRE3G Promoter#Nucleotide Barcode#CMV Promoter#ZsGreen#Puromycin Resistance
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