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GFP-TRAP in HCT116 cells transfected with 82bd-GFP or 82bdDPR-AAA-GFP. The blot is probed with antibodies to GFP and WDR82. Quantitation shows the IP efficiency of WDR82 for the DPR mutant versus the unmodified fragment after normalizing to GFP signal. n = 3.
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