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Schematic of the complementation strategy. Endogenous ZC3H4 (green) is tagged with DHFR (gray) and is degraded by withdrawing TMP (pink) from culture media. Stably integrated FLAG-tagged ZC3H4 derivatives (blue) are induced by dox. Western blot exemplifies this in the WT ZC3H4 complementation cell line. The presence/absence of dox (16 h)/TMP (4 h) is indicated. FLAG-tagged ZC3H4, ZC3H4-DHFR, and the nucleolin loading control are blotted. ZC3H4-DHFR (blue asterisk) is larger than ZC3H4-FLAG (red asterisk).

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