Quantification of steady-state tubulin mRNA levels in the indicated HEK T-REx cell lines. Tubulin mRNA levels were normalized to a reference gene (RPLP1) and to the WT cell line, and data from all relevant experiments in the manuscript were compiled. Data show mean SD. Statistical analysis for SCAPER-KO and rescue cell lines was performed using a one-sample t test. Values significantly different from 1 (WT levels) are indicated by double and triple asterisks (p < 0.01, and p < 0.001, respectively; ns indicates not significant). n = 11 for WT and SCAPER-KO, n = 8 for KO + WT; data reanalyzed fromFigures 3E, 3F,5D, andS4D. See alsoFigure S9.
