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K+-flux assay using proteoliposomes containing TMEM175 or TMEM175/LAMP complexes. K+flux was initiated by adding H+ionophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) and terminated by adding K+ionophore valinomycin (Val). The assay was repeated 3 times with consistent results. See alsoFigure S3.
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