Gel from Scientific Research

In vitro ADP-ribosylation assay showing ability of E. coli C7 DarT1 to ADP-ribosylate all guanosine sites in the DarT-ADPr-27-mer, yet with no double modification of the AGGC site. The assay uses an oligo with four bases around the to-be-tested guanosine of the DarT-ADPr-27-mer flanked by random guanosine-free sequences, considering a four-base motif with the targeted nucleotide in third position as for DarT2 activity.

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