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Scheme of the mating competition assay of 1-day-old male mosquitoes (30 females were paired with 30 males per group). The ratios of LAP1 -/- male to WT male were 0:1 (0 LAP1 -/- male and 30 WT male), 1:1 (15 LAP1 -/- male and 15 WT male), 2:1 (20 LAP1 -/- male and 10 WT male), 5:1 (25 LAP1 -/- male and 5 WT male), 9:1 (27 LAP1 -/ - male and 3 WT male), 14:1 (28 LAP1 -/- male and 2 WT male), and 29:1 (29 LAP1 -/- male and 1 WT male) for a total of 30 males in each group.

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