The fertility of WT females mated with LAP1 -/- and WT males at different ratios. 6-day-old LAP1 -/- and 1-day-old WT males were introduced simultaneously at different ratios (30 males in total) to mate with 30 WT females ( n = 64 for 0:1; n = 58 for 1:1; n = 59 for 2:1, 5:1 and 14:1; n = 62 for both 9:1 and 29:1). Blood meal was given after 3 days. And egg hatchability was measured. Females that died during laying eggs were removed from the study. The top of each column in b and e shows the number of hatched mosquitoes relative to total mosquitoes. Each dot represents one mosquito. The experiments were independently performed three times with 30 females per experiment. A Kruskal–Wallis test of variance was conducted to detect any significant variation among three replicates, and no significant differences were detected. Then results were combined for further analyses. Statistical significance of hatchability was determined using the Kruskal–Wallis test followed by Dunn’s post-hoc tests with Benjamini–Hochberg adjustment for multiple comparisons ( b and e ). The q -value was calculated by comparing the experimental group with the 0:1 group. Data are shown as mean ± SEM. * q < 0.05, ** q < 0.01, *** q < 0.001. Source data are provided as a Source Data file.
