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Temporal expression of LAP1 during different stages of embryonic development in LAP1 −/− ♂ × WT♀ and WT♂ × WT♀ groups ( n = 3). The experiment was repeated three times, and total RNA was extracted from eggs collected from the pairs LAP1 −/− ♂ × WT♀ and WT♂ × WT♀ at different periods (0–1, 1–2, 2–4, 4–6, 6–8, 8–10, 10–12, and 20–22 h) after the first and second blood meals as individual replicates for each group. RNA level of LAP1 was measured using qPCR. Data were normalized to the expression level of WT♂ × WT♀ at 0–1 h post first blood meal. Statistical significance was determined using the two-sided multiple Mann–Whitney test with Benjamini–Hochberg adjustment. ns: not significant. Data in a , c and d are represented as mean ± SEM. The experiments were repeated three times with similar results. Source data are provided as a Source Data file.
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