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Diagram shows PAINT 3.0-mediated targeted integration of a CD19 CAR-EGFP transgene cassette at the TRAC locus in primary T cells. Yellow triangle marks the genomic target site recognized by sa-sgTRAC. Red and cyan lines indicate the 800-bp left HA and the 35-bp right MH, respectively. Cyan triangle indicates the spacer targeted by pega. Precisely edited cells express CD19 CAR and EGFP under the regulation of the TCR promoter. Positions of primers for genotyping are shown by arrows.

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