Diagram shows HDR-, NHEJ-, MMEJ-, and PAINT-mediated T2A-EGFP integration at the 3′-UTR of GAPDH . The yellow triangle marks the genomic target site recognized by sgGAPDH. The long red and cyan lines indicate the 800-bp left and right HAs, respectively. The short red and cyan lines indicate the left and right MHs (35 bp for PAINT and 20 bp for MMEJ), respectively. There is a 59-bp distance between the integration target site and the genomic cleavage site. The red and cyan triangles indicate the spacers targeted by sgα (or pegα) and sgγ (or pegγ), respectively. Precisely edited cells express EGFP in-frame of the GAPDH coding sequence.
