Diagram shows HDR-, NHEJ-, and PAINT-mediated integration of an IRES-EGFP reporter transgene at the 3'-UTR of human GAPDH gene. The yellow triangle marks the genomic target site recognized by sgGAPDH. The long red and cyan lines indicate the 800-bp left and right homologous arms (HAs), respectively. The short red and cyan lines indicate the 35-bp left and right micro-homologues (MHs), respectively. The red and cyan triangles indicate the generic spacers targeted by sgα (or pegα) and sgβ (or pegβ), respectively. PegRNAs are designed corresponding to the MHs and spacers. Correctly edited cells express EGFP and can be observed under a fluorescence microscope or analyzed by FACS. Positions of primers for PCR genotyping are shown by arrows.
