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Quantification of cellular IFNB1 mRNA in WT, UBR5 -/- (made with gRNA #1) and IFIH1 -/- (gene symbol for MDA5) HEK293T cells transfected with poly (I:C) for 12 h. Data presented in mean ± S.E.M, ordinary one-way ANOVA with Dunnett’s test, n = 3 biological independent experiments; for : *** p = 0.0001, **** p < 0.0001 vs WT. Multiplicity adjusted p values are presented.

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