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Immunoblot analysis of intracellular H3pT11 levels in the mutants of the indicated telomere-binding factors. WT TetO 7 , TetO 7 - CDC13 , TetO 7 - TEN1 and TetO 7 - RFA2 mutants were grown in YPD medium at 30 C until OD 600 of 0.5–0.7 and then treated with 40 g/mL doxycycline for 2 h to knockdown the expression of CDC13 , TEN1 and RFA2 , respectively. The knockdown efficiency results were shown in Supplementary Fig. S4b .

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