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ChIP-qPCR analysis of the occupancy of Glc7 ( i ) and H3pT11/H3 ( j ) at ATG genes in WT TetO 7 and TetO 7 - SEN1 mutant. For f – j , WT TetO 7 and TetO 7 - SEN1 mutant were grown in YPD medium at 30 C until OD 600 of 0.5–0.7 and then treated with 40 g/mL doxycycline for 2 h to knockdown the expression of SEN1 . Data represent the mean SE of three biological independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

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