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Immunoblot analysis of intracellular H3pT11 levels in WT TetO 7 , TetO 7 - SEN1 , TetO 7 - NRD1 and TetO 7 - NAB3 mutants. WT TetO 7 , TetO 7 - SEN1 , TetO 7 - NRD1 and TetO 7 - NAB3 mutants were grown in YPD medium at 30 C until OD 600 of 0.5–0.7 and then treated with 40 g/mL doxycycline for 2 h to knockdown the expression of SEN1 , NRD1 and NAB3 , respectively. The knockdown efficiency results were shown in Supplementary Fig. S4b .
#Bar Plot#Error Bars#Gel#Immunoblot Analysis#H3pT11#WT TetO7#SEN1 Mutants#NRD1 Mutants#NAB3 Mutants#Doxycycline#Knockdown
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