Immunoblot analysis of the intracellular H3pT11 levels in 44 protein phosphatase mutants. The relative intensities of H3pT11/H3 were quantified using Image J with standard error (SE). Yeast cells were grown in YPD medium at 30 C until OD 600 of 1.0. To inactivate Glc7, WT and glc7-12 mutant were grown at 26 C until OD 600 of 0.5 and then treated at 37 C for 2 h. For WT TetO 7 , TetO 7 - CDC14 , and TetO 7 - SSU72 , cells were grown in YPD medium at 30 C until OD 600 of 0.5–0.7 and then treated with 40 g/mL doxycycline for 2 h to knockdown the expression of CDC14 and SSU72 , respectively. For a , data represent the mean SE of three biological independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.
