RT-qPCR analysis of IFNB1 mRNA expression in WT (k and l) or FUT8KO (j and l) HEK293 transiently transfected with indicated plasmids or not for 48 h, and then infected with VSV (MOI = 0.001) or SeV (MOI = 0.01) for 12 h (j – l, n = 3 per group per study). Data are normalized based on GAPDH for a, b, and d – l. Data in all quantitative panels are presented as mean ± SD. Data are representative of three independent experiments. Two-tailed unpaired Student’s t test was used to assess the statistical difference in a, b, d – l. Source data are provided as a Source Data file.
