Microscopy from Scientific Research

Live-cell imaging of dendritic F-actin before (initial), during (frames 1-15) and after (final) application of a stimulation solution (0 Mg 2+ /Gly/2.4 mM Ca 2+ ). Shown are the confocal (red, top row), synthetic (purple, middle row) and real (orange, middle row) STED images when acquired, and corresponding segmentation masks for F-actin fibres (magenta, bottom row). The series was chosen as a representative example from a total of 72 series. Colour bars: raw photon counts.

DOI