EMSA of SNAIL binding to the promoter of FUT8. The purified recombinant His-SNAIL protein was incubated with probes, and the protein-DNA complexes were separated on native polyacrylamide gels. “−“ represents absence and “+” represents presence. Unlabeled or mutant probes at 300 nm were added to the reaction mixture for competition and testing binding specificity. Arrows indicate the positions of shifted bands.
