Top: self-assembly of an origami mix with staples coding for sharp triangles, including biotinylated staples at specific positions a-f, in the presence of 2 M streptavidin (Strep). Middle: representative AFM images of the obtained origamis after 24 h of assembly in TANa ([NaCl] = 100 mM), as a function of the biotinylated staples used in the mix. Larger-scale images are given in Supplementary Fig. 16. Bottom: fraction (%) of streptavidin detected on origamis functionalized with 1 to 6 streptavidin proteins. The box plots display a box (orange) ranging from the first to third quartiles (black bars), with extrema indicated by shorter black bars and the median shown as a red bar. Each of the n counted streptavidins is represented by a cross. All images used for these analyses are available in a citable public repository (doi: 10.5281/zenodo.7998757) and can be accessed directly at the following link: https://zenodo.org/record/7998757. [M13] = 1 nM; each staple concentration is 40 nM.
