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Overall tyramine excision (TyrEx) strategy for labelling of His6-PlpA3-M5G: PlpXY expressed in the cell installs aminopyruvate site-specifically in the target biomolecule at a GYG-containing motif. Splicing results in a mass loss of -135.0757 Da (-C8H9NO). The post-translationally modified product reacts tetrazine derivatives to form the pyridazyl protein conjugates a – c. Structures are based on models calculated with AlphaFold 47, 48.

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