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Reaction kinetics in PBS pH 7.4 measured by visible light spectrometry. The decrease in absorbance at 515 nm of tetrazine 2 was measured over time and fitted to a second-order reaction kinetic equation yielding an apparent rate constant of k obs = 0.625 (15) M -1 s -1 in 30% DMSO in H 2 O (n = 3 independent experiments). Points describe mean; error bars describe standard deviation.
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