Line Plot from Scientific Research

Time constants of duration times of the main pause (left) and the sub-pause (right) at different [ATP]s for the wild-type V1 (black), the designed V1 (red) and the design double mutant K157A/S158A (magenta). The time constants were plotted with values obtained by analysing all detected pauses for three molecules and the error bars represent the s.d. See Supplementary Figs. 14 and 15 for distributions of the duration time. Note that for the main pauses at 1003,000 M ATP, two different time constants were obtained for each [ATP] assuming consecutive reactions (Supplementary Fig. 14).

DOI