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Generation of Fab HER2 –Fab CD3 –biotin 12 . Fab HER2 –BCN 10 was reacted with Fab CD3 –Tet–N 3 14 to form Fab HER2 –Fab CD3 –N 3 13 . This construct was then reacted with DBCO–biotin 5 to generate Fab HER2 –Fab CD3 –biotin 12 after SEC purification.
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