OTU richness measured in the six crop rotations from 2016 to 2022. The percentage values represent the increment from 2016 to 2022; dot is the mean value, whisper line bars on each dot are the standard deviations for three replications. One-way ANOVA with two-sided and post-hoc test was conducted to determine significant differences among treatments. Different lowercase letters below pillars indicate significant differences between rotations at P < 0.05.